Biomedical labs
Key equipment
Flow Cytometry
Flow cytometry is a technique for counting and examining microscopic particles, such as cells and chromosomes, by suspending them in a stream of fluid and passing them by an electronic detection apparatus. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of particles per second.
Equipment:-
BD FACSVerse
* Laser: 2
* 488nm (blue)
* 640nm (red)
* Software: BD FACSuite
Data analysis software:-
ModFit LT Version 4.0 - DNA analysis software by Verity Software House
Real Time PCR systems
StepOnePlus™ Real-Time PCR System (left pic below) is a 96-well Real-Time PCR instrument that utilize robust LED based 4-colour optical reading to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.
iQ5 real-time PCR detection system BioRad (right pic) is designed for multiplex analysis, enabling reproducible quantitation of up to 5 targets simultaneously.
High Performance Liquid Chromatography (HPLC)
Analytical HPLC
Agilent 1200 – with MWD (left pic) and Agilent 1200 – with DAD and FLD (right pic)
Analytical HPLC is used to determine the existence of compound(s) of interest in a sample. User can also quantify its amount using this technique.
Equipment:-
Agilent 1200 Series
* Pump: Quaternary
* Detector
– DAD
– MWD
– FLD
* with autosampler, thermostated column compartment and fraction collector
Preparative HPLC
Preparative HPLC is used to isolate and purify of compound(s) of interest for further use.
Gilson PLC 2020 Agilent 1260 Infinity
The Agilent 1260 Infinity (pic above) has one quaternary pump and two preparative pumps
* Detector: DAD
* with autosampler, thermostated column compartment and fraction collector
Chromatography – Protein Purification System
ÄKTA Prime plus
This is a low pressure chromatography system that performs simple purifications of tagged and untagged proteins. The system can be used for general purification tasks such as buffer exchange, gel filtration, ion exchange, HIC and affinity chromatography.
ÄKTA Purifier UPC 100
This system is widely used for rapid purification of proteins at low to medium pressure. This system allows UV-Vis detection of up to three wavelengths simultaneously.
Agilent 3100 OFFGEL Fractionator
OFFGEL electrophoresis is a pI-based fractionation of proteins and peptides with liquid-phase recovery.
PhastSystem Separation-Control and Development Units
PhastSystem is a multi-tasking, semi-automated electrophoresis workstation. It consists of a separation-control unit for system control and electrophoresis, and also a development unit for gel staining.
GE Healthcare Ettan IPGphor 3
The Ettan IPGphor 3 is a fully integrated isoelectric focusing (IEF) system optimized to deliver high throughput, ease of use, speed, reproducibility, and high protein-loading capacity. It can run up to 12 IPG strips (7, 11, 13, 18, or 24 cm) simultaneously using Ettan IPGphor manifold, Ettan IPGphor Manifold Light, or ceramic strip holders.
GE Healthcare SE600 Vertical Unit and Ettan DALTsix Large Vertical System
These two setup can run denaturing and native polyacrylamide, 2-D, electrophoresis, agarose, and nucleic acid gel electrophoresis. The SE600 is capable to run two gels simultaneously-or run four gels using accessory divider plates. Meanwhile, the Ettan DALTsix system can run up to six lab-cast or precast gels (26 × 20 cm) simultaneously.
Gel Imaging System
High-resolution chemiluminescence and fluorescence imaging.
Molecular Imager® ChemiDoc™ XRS System Vilber Lourmat E-Box VX2 
Imagemaster Platinum analysis software
GE Healthcare Life Sciences
ImageMaster Plantinum analysis sofware is design for the comprehensive visualization, exploration, and analysis of 2-D gel data from both 2-D DIGE and non-DIGE technologies.
BX41, CKX41 and IX81 Microscope
The BX41 is a clinical microscope which integrates brightfield, fluorescence, phase contrast, polarization and darkfield modulars. The CKX41 is an inverted microscope. It is routinely used for checking cell viability in petri dishes, culture flasks, and multi-well plates. The IX81 represents the most advanced motorized inverted microscope from Olympus. It is ready for multi-wavelength, advanced fluorescence and deconvolution techniques.
BX41 and CKX41 microscopes use the AnalySIS LS Report 2.6 software to capture image digitally. IX81 microscope can be controlled electronically by using the control board. Alternatively, it can also be controlled by using CellSens Dimension 1.6 Software (pictures below).
Fluorescence / Chemiluminscence Spectrophotometer
Perkin Elmer VICTOR™ X5 Multilabel Plate Reader
This multilabel, multitask plate reader is capable to run multiple assay technologies on a single platform. It is equipped with Luminescence, Fluorescence Intensity (both top and bottom reading), UV-absorbance (UV-VIS), Time-Resolved Fluorescence and Fluorescence Polarization technologies.
ELISA Reader
BIO-RAD Benchmark Plus Microplate Reader
This reader provides all the flexibility required for absorbance assays. It has the capability to perform standard colormetric ELISA and kinetic readings for general gel screening with a range microplate formats. It also provide an optional incubation feature with the temperature range up to 45oC.
DNA & Protein Analyser
Bio-Rad Experion Automated Electrophoresis System
This system employs LabChip microfluidic technology to automate protein and nucleic acid electrophoresis. It integrates separation, detection and data analysis within a single platform.
CHEF-DR® III Pulsed Field Electrophoresis System
Pulsed field electrophoresis is a technique used to resolve chromosome-sized DNAs. By alternating the electric field between spatially distinct pairs of electrodes, megabase sized DNAs are able to reorient and move at different speeds through the pores in an agarose gel. DNAs from 100 bases to over 10 megabase may be effectively resolved.
High Speed Centrifuge
Beckman Coulter OPTIMA™ L-100 XP Ultracentrifuge
The centrifuge is capable of generating 801400 x g at speeds up to 100000 rpm.
Beckman Coulter Allegra X-30R centrifuge (refrigerated) – located in CSJB
Features:-
* Speed of up to 18,000 rpm (in 100 rpm increment)
* Temperature can be set ranged from -20ºC to 40°C
* Acceleration and deceleration of 10 acceleration/deceleration profiles
* Rotor available:-
– F2402H Hermetically-Sealed Rotor: It can centrifuge up to 24 1.5 -2.0mL tubes as well 250-750µL vials.
– SX4400 Swinging Bucket Rotor: A four-place swinging bucket rotor that carries adapters that allow centrifugation of a wide range of tube and bottle sizes (from 1.5mL reaction vials to 250mL bottles).
Freeze Dryer
Freeze dryer is used to remove solvent from the sample/product by sublimation. One of the main functions of freeze drying is to stabilize delicate samples. (eg. proteins, peptides, etc.)
ScanVac Cool Safe Freeze Dry System Labconco FreeZone 4.5 Freeze Dry System
Eyela FDU-1100 Freeze Dryer
