Bioimaging Lab
Bioimaging is a tool to create structural or functional images of living objects or systems at the levels of tissues, cellular, subcellular and molecular structures. Bioimaging is an essential technology in a variety of research field including structural biology, molecular biology, biochemistry, pharmacology, agricultural sciences, biomedical science and neuroscience (=neuroimaging). Neuroimaging allow the non-invasive analysis of brain anatomy as well as function in living animals, thus opening up unprecedented ways of exploring brain function. Neuroimaging is one of the most critical and essential technology in Brain Science as well as in Psychological Disease Research.
Collaborators
- UPM
- IMCB (Singapore)
- Cancer Research Malaysia
- Sun Yet-Sen University (China)
- The Hebrew University of Jerusalem (Israel)
- Niigata University (Japan)
- Monash University (Clayton/Parkville)
- Chinese Hong Kong University (Hong Kong)
Industrial Partner
Hi-Tech Instruments Sdn Bhd
Equipment
Upright Bright Field Microscope
→ Conventional upright bright field microscope with a high resolution color digital camera, monitor, camera control unit
→ Observation and image capturing of bright field sample slides
Upright Fluorescence Microscope
→ High-end upright bright field and fluorescent microscope with motorized objectives, fluorescent filter wheel and shutter, a high resolution cooled color CCD camera, PC unit with controlling software. Obervation, image capturing and image data analysis of bright field and fluorescence samples.
Inverted Fluorescence Microscope
→ High-end inverted bright field and fluorescent microscope with motorized shutter, a high resolution cooled color CCD camera, PC unit with controlling software. Obervation, image capturing and image data analysis of bright field and fluorescence samples for culture cells.
Live cell imaging system
Advanced fluorescence imaging system, which is ideal for applications in fluorescence microscopy and image analysis including live cell time-lapse experiments, multi-positioning, z-stacking and deconvolution of variety of samples including cell and tissue culture.
Laser confocal microscope
→ A spectral imaging confocal laser microscope system with the capability to acquire 32 channels of fluorescence spectra over a 320 nm wide wavelength range in a single pass, which also eliminates spectral crosstalk (unmixing of images). The system allows to perform wide range of imaging of cell culture and tissue sections including 3D-imaging, FRET assay, time-lapse recording.
Digital slide scanner
→ Computer controlled, automated slide imager (up to12 slides per run). Creates stitched composites of entire slides as a single image (0.23 μm resolution).
Laser capture microdissection microscope
→ It has a proprietary combination of a gentle IR laser and a powerful UV laser that work in conjunction to isolate cells without changing morphology or integrity of the biological content. The IR laser helps to capture the cells of interest, and the UV laser microdissects the captured cells.
Multiphoton microscope
→ A multiphoton microscope makes use of a long wavelengths infrared (IR) lasers up to 1300 nm (Insight DeepSee), including prechirped femtosecond lasers the high efficiency of HyD detectors, and an infrared-optimized optical transmission, which allows to deeply penetrate into the tissue with super-sensitivity and to uncover the finest details of cellular and subcellular processes.
Cryostat
→ A machine for sectioning of fresh/fixed frozen tissue specimens (1-100um) for routine histology and clinical pathology
Vibratome
→ A machine for sectioning of fresh/fixed tissue specimens (30-500um) for routine histology and tissue culture
Microtome
→ A machine for sectioning of fixed paraffine embedded tissue specimens (1-100um) for routine histology and clinical pathology
Stereoscopic microscope
→ The stereo or stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.
Micropipette Puller
→ A system for fabricating glass micropipettes, patch pipettes and microinjection needles.
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Fluorescence StereoZoom Microscope
→ This Fluorescence StereoZoom Microscope (Model: Carl Zeiss, Axio Zoom V16) is equipped with three fluorescent filters (blue, green, and UV), a monochrome digital camera and digital imaging workstation (ZEN 3.0 Pro) that can be used to observe small model organisms (eg: whole fish larvae, rodents embryos,etc).
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