Dr Muhamad Noor Alfarizal Bin Kamarudin

Research Fellow

Jeffrey Cheah School of Medicine & Health Sciences

Email: MuhamadNoor.Alfarizal@monash.edu
Tel+603 5514 6000 (Ext: 44986)
Office: Room 3-3-03

ORCID ID: https://orcid.org/0000-0003-2505-1018

Profile Summary

Dr Muhamad Noor Alfarizal Kamarudin is a research fellow at Brain Research Institute Monash Sunway (BRIMS), Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia. He is undertaking research on with special interest to unravel the novel and intriguing role of RFamides as therapeutic targets in Glioblastoma multiforme. Additionally, his research interest also lies on the complex regulation of inflammation in neurodegenerative diseases.

He completed his Bachelor Degree (Biochemistry, Hons) from University of Malaya (UM) and pursued his Masters studies of which he successfully converted into a doctorate program (UM, 2012) with specialization in molecular biology, neuroimmunology, neuroprotection, cancer biology and natural products. His PhD thesis focuses on the modulation of molecular signaling in neuroinflammation with special attention to cytokines and chemokines where he identified a potential novel role of CCL21/exodus in neuronal differentiation and key regulator of microglia activation. Through this, he developed an immense interest and in-depth knowledge on the modulation of neuroinflammation, particularly innate immunity in neurons and glia cells in neuroprotection, neuritogenesis and anti-inflammatory.

Upon completing his PhD (2016), he obtained a postdoctoral researcher position under a three way collaboration between UM, Taibah University of Medina and Lincoln College University on Glioblastoma multiforme (GBM) project. He is also an active reviewer for several journals such as Journal of Ethnopharmacology and Journal of Functional Food.

He has been involved and hold the key role in obtaining various research grant such as High Impact Research (HIR) UM, Fundamental Research Grant Scheme (MoHE – Best FRGS Project Award 2014) and University Malaya Research Grant (UMRG).

Academic Qualifications

  • Doctor of Philosophy (Biochemistry), University of Malaya
  • Bachelor of Science (Biochemistry, University of Malaya – Best Thesis Award, University of Malaya
  • Associate Diploma (Instrument Studio Teaching), International Music of Exam Board, Australia.

Research Experience & Employment

  • Oct 2017 – present Research Fellow, Brain Research Institute Monash Sunway (BRIMS), Jeffrey Cheah School of Medicine, Monash University Malaysia.
  • 2016 – 2017 Post-doctorate, Lincoln University College
  • 2014 – 2016 Research Assistant, Biomolecular Research Group (University of Malaya)
  • 2010 – 2013 Research Assistant, Biomolecular Research Group (University of Malaya)

Thesis

  • PhD Thesis (2012 - 2016)
    Title of Thesis: (R)-(+)-α-lipoc acid and mesuagenin c-induced regulation of NF-κB, cytokines and chemokines via PI3K-AKT/GSK-3β and ERK1/2 in in vitro neuronal models.
    Short  description:  The investigation  on  the protective  and  neuritogenic effects  of  the natural products in neuronal models (neurons, microglia and neuron-glia) in regulating innate immunity by PI3K-AKT/GSK-3β  and  ERK1/2. The work discovered the possible novel role CCL21 in neurite outgrowth and differentiation.
  • Master (2011- 2012, Converted to Ph.D)
    Title  of Thesis:  Mesuagenin  c mediated  neuroprotective  and neuritogenic  in  neuronal models through cytokines and chemokine via regulation of NF-κB.
  • Bachelor (2010)
    Title of Thesis: Structural-Activity Analysis of Indolic Gallic  Acid Hydrazones Derivatives on Human  Breast Adenocarcinoma  Cell  Line (MCF-7)  and  Human Colon  Adenocarcinoma  Cell Line (HCT-119)
    This thesis has been conferred with Best Thesis Award & Amersham Biosciences Award by the Malaysia Society of Biochemistry and Molecular Biology (MSBMB).

Journal Papers in Preparation

  • Kamarudin,  M.  N.  A. & Parhar, I. S. (2018).  The emerging therapeutic potential of anti- psychotics drugs in the management  of human gliomas. (Review  Article, in preparation for Neuro-oncology).
  • Kamarudin, M. N. A., Parhar, I. S., & Sarker, M. M. R. (2018). Is metformin effective for the treatment of colorectal cancer? A review on its molecular mechanism, pre-clinical and clinical aspects. (Oncotarget).
  • Sheikh, B. Y., Kamarudin, M. N.  A., Parhar, I. S., Sarker, M. M. R., & Mohan, G. D.M. (2016). Oleic acid induces growth inhibition and ROS-mediated mitochondrial-mediated apoptosis in  Ca Ski cells. (Prostaglandins, Leukotrienes and Essential Fatty Acids).

Personal Interest

Orchestra conducting - Principal Conductor for Alam Shah Wind Orchestra with various National and International Awards.

Research Expertise

  • Cancer biology – Gliomas & Glioblastoma multiforme
  • Molecular Biology
  • Neuroimmunology & Neuroprotection
  • Molecular signal transduction – Protein Signaling
  • Nutraceuticals

Research Interests

Dr Muhamad Noor Alfarizal Kamarudin's main research aspiration is to develop novel target in human gliomas management that will tackle the issue of chemoresistant and tumor recurrence which ultimately will improve the prognosis among patients with different tumor status and background. In doing so, he believes that the cardinal focus should be on multimodal mechanistic pathways by using natural products and the modulation of alternative neuroendocrine system. He envisions the bright future of human gliomas management through their great potentiality as neo-adjuvants and/or chemotherapeutics while being cost-effective with marginal side-effects would lead to a more affordable treatment in the current setting of gliomas healthcare.

Areas of Interests:

  • Molecular Biology & Diseases Mechanism
  • Neuroimmunology (Neuroprotection, Neuroinflammation) & Neuronal Differentiation)
  • Neurooncology & Cancer biology
  • Molecular signal transduction – Protein Signaling
  • Nutraceuticals

Current Research Projects

  • The modulation of neuroendocrine and their alternative pathways as target sites in high grade gliomas.
  • Lentinan and 6-shogaol as chemosensitizer agent of temozolomide in human glioblastoma multiforme.

Publications

Peer reviewed Journal Papers

Chan, C. K., Tan, L. T. H., Andy, S. N., Kamarudin, M. N. A., Goh, B. H., & Kadir, H. A. (2017). Anti-neuroinflammatory Activity of Elephantopus scaber L. via Activation of Nrf2/HO-1 Signaling and Inhibition of p38 MAPK Pathway in LPS-Induced Microglia BV-2 Cells. Frontiers in Pharmacology, 8, 397.

Sheikh, B. Y., Sarker, M. M. R., Kamarudin, M. N. A., & Mohan, G. (2017). Antiproliferative and apoptosis inducing effects of citral via p53 and ROS-induced mitochondrial-mediated apoptosis in human colorectal HCT116 and HT29 cell lines. Biomedicine & Pharmacotherapy, 96, 834-846.

Kamarudin, M. N. A., Sarker, M. M. R., Kadir, H. A., & Ming, L. C. (2017). Ethnopharmacological uses, phytochemistry, biological activities, and therapeutic applications of Clinacanthus nutans (Burm. f.) Lindau: A comprehensive review. Journal of Ethnopharmacology.

Sheikh, B. Y., Sarker, M. M. R., Kamarudin, M. N. A., & Ismail, A. (2017). Prophetic medicine as potential functional food elements in the intervention of cancer: A review. Biomedicine & Pharmacotherapy, 95, 614-648.

Supriady, H., Kamarudin, M. N. A., Chan, C. K., Goh, B. H., & Kadir, H. A. (2015). SMEAF attenuates the production of pro-inflammatory mediators through the inactivation of Akt-dependent NF-κB, p38 and ERK1/2 pathways in LPS-stimulated BV-2 microglial cells. Journal of Functional Foods, 17, 434-448.

Syed Hussein, S. S., Kamarudin, M. N. A., & Abdul Kadir, H. (2015). (+)-Catechin Attenuates NF-κB Activation Through Regulation of Akt, MAPK, and AMPK Signaling Pathways in LPS-Induced BV-2 Microglial Cells. The American Journal of Chinese Medicine, 43(05), 927-952

Lo, J. Y., Kamarudin, M. N. A., Hamdi, O. A. A., Awang, K., & Kadir, H. A. (2015). Curcumenol isolated from Curcuma zedoaria suppresses Akt-mediated NF-κB activation and p38 MAPK signaling pathway in LPS-stimulated BV-2 microglial cells. Food & function, 6(11), 3550-3559.

Hamdi, O. A. A., Ye, L. J., Kamarudin, M. N. A., Hazni, H., Paydar, M., Looi, C. Y., ... & Awang, K. (2015). Neuroprotective and Antioxidant Constituents from Curcuma zedoaria Rhizomes. Records of Natural Products, 9(3), 349.

Moghadamtousi, S. Z., Kamarudin, M. N. A., Chan, C. K., Goh, B. H., & Kadir, H. A. (2014). Phytochemistry and biology of Loranthus parasiticus Merr, a commonly used herbal medicine. The American journal of Chinese medicine, 42(01), 23-35.

Kamarudin, M. N. A., Raflee, N. A. M., Hussein, S. S. S., Lo, J. Y., Supriady, H., & Kadir, H. A. (2014). (R)-(+)-α-Lipoic acid protected NG108-15 cells against H2O2-induced cell death through PI3K-Akt/GSK-3β pathway and suppression of NF-κβ-cytokines. Drug design, development and therapy, 8, 1765.

Goh, B. H., Chan, C. K., Kamarudin, M. N. A., & Kadir, H. A. (2014). Swietenia macrophylla King induces mitochondrial-mediated apoptosis through p53 upregulation in HCT116 colorectal carcinoma cells. Journal of ethnopharmacology, 153(2), 375-385.

Chan, G., Kamarudin, M. N. A., Wong, D. Z. H., Ismail, N. H., Abdul Latif, F., Hasan, A., ... & Abdul Kadir, H. (2012). Mitigation of H2O2-induced mitochondrial-mediated apoptosis in NG108-15 cells by novel mesuagenin C from Mesua kunstleri (King) kosterm. Evidence-Based Complementary and Alternative Medicine, 2012.

Chan, C. K., Goh, B. H., Kamarudin, M. N. A., & Kadir, H. A. (2012). Aqueous fraction of Nephelium ramboutan-ake rind induces mitochondrial-mediated apoptosis in HT-29 human colorectal adenocarcinoma cells. Molecules, 17(6), 6633-6657.

Proceeding/Conference Abstracts

Mesuagenin C attenuated LPS-stimulated BV-2 and NG108-15 cells by modulating  NF-κB,  CCL21 and  cytokines through PI3K-  Akt/GSK-3β. FEBS  EMBO 2014,  Palais des  Congrès  in Paris,  France,  August 30-September  4,  2014. Special  Issue: FEBS EMBO 2014 Conference, FEBS Journal, 281(s1), 1(823).

Protective effects of (R)-(+)-α-Lipoic Acid against MPP+-stimulated microglia  cells  and toxicity in  dopaminergic  SH-SY5Y cells  through  PI3K-Akt/GSK- 3β pathway. FEBS EMBO 2014, Palais des Congrès in Paris, France, August 30-September 4, 2014. Special Issue: FEBS EMBO 2014 Conference, FEBS Journal, 281(s1), 1(823).

Catechin supressed  LPS-induced  neuroinflammation  in BV-2,  C6 and NG108-15 cells by modulating CCL21 through PI3K-Akt. Journal of the Neurological Sciences, 333, e337.   XXI World Congress of Neurology held at Reed Messe Wien GmbH Congress Center, Vienna, Austria, September 21-26, 2013.

α-Lipoic  acid  protects against  LPS-induced  BV-2 activation  and MPTP- induced toxicity in SH-SY5Y neuronal cells. Journal of the Neurological Sciences, 333, e338. XXI World Congress of Neurology held at Reed Messe Wien GmbH Congress Center, Vienna, Austria, September 21-26, 2013.

Mesuagenin C mitigated  LPS-induced  neuroinflammation in BV-2 and  NG108-15 cells  through  pMTOR-PI3K-Akt  and CCL21  downregulation.  Journal of the  Neurological  Sciences, 333,  e349.  XXI World  Congress  of Neurology  held  at Reed Messe Wien GmbH Congress Center, Vienna, Austria, September 21-26, 2013. 2013.

Conference Presentation

Oral paper presentation

Mesuagenin c suppresses microglia inflammation and improves neuronal   survival   by modulating   NF-Κb,   cytokines and   chemokines   through PI3K- Akt/GSK-3β. International Post-Graduate Seminar University of Malaya 2014.

Neuroprotective and  neuritogenic  molecular mechanisms  of novel   mesuagenin C   from   Mesua kunstleri   (King)   Kosterm in      NG108-15   cells. Symposium  on Natural  Products  and Medicinal  Chemistry  (NPMC), 17th  Malaysian Chemical Congress (17MCC),  October  15- October  17,  2012, Putra  World  Trade Centre, Kuala Lumpur, Malaysia.

Neuroprotective and  neuritogenic  molecular mechanisms  of mesuagenin  C from  Mesua   kunstleri through  CCL21  regulation in  NG108-15   cells. International Conference on Molecular Biology and Biochemistry, WASET. May 28 – Jun 1, Chiba, Tokyo, Japan.

Poster  presentation:

Neuroprotection and neuritogenesis effects of Mesuagenin C from Mesua kunstleri in NG108-15 cells. The 1st World Congress on Healthy Ageing 2012, 19th to 22nd March 2012 at Kuala Lumpur Convention Centre.

α-Lipoic  acid  mitigated H2O2-induced mitochondrial-mediated apoptosis in NG108-15 cells through PI3K-AKT pathway.  24th Intervarsity Biochemistry Seminar, 11 May 2013 to 11 May 2013, Taylor's University College & Malaysian Society of Biochemistry and Molecular Biology (MSBMB).

Swietenia macrophylla  ethyl  acetate fraction  suppressed  LPS- induced neuroinflammation  in  BV-2 cells  through  PI3K/Akt and  MAPK  pathways. 39th Annual  Conference  of The  Malaysian  Society for  Biochemistry  & Molecular  Biology, 25th-26th June 2014, Sama-Sama Hotel, KLIA, Sepang, Malaysia.

Mesuagenin C attenuated LPS-stimulated BV-2 and NG108-15 cells by modulating  NF-κB,  CCL21 and  cytokines through PI3K-  Akt/GSK-3β. FEBS  EMBO 2014,  Palais des  Congrès  in Paris,  France,  August 30-September  4,  2014. Special  Issue: FEBS EMBO 2014 Conference, FEBS Journal, 281(s1), 1(823).

Protective effects of (R)-(+)-α-Lipoic Acid against MPP+-stimulated microglia  cells  and toxicity in  dopaminergic  SH-SY5Y cells  through  PI3K-Akt/GSK- 3β pathway. FEBS EMBO 2014, Palais des Congrès in Paris, France, August 30-September 4, 2014. Special Issue: FEBS EMBO 2014 Conference, FEBS Journal, 281(s1), 1(823).

Catechin supressed  LPS-induced  neuroinflammation  in BV-2,  C6 and NG108-15 cells by modulating CCL21 through PI3K-Akt. Journal of the Neurological Sciences, 333, e337.   XXI World Congress of Neurology held at Reed Messe Wien GmbH Congress Center, Vienna, Austria, September 21-26, 2013.

α-Lipoic  acid  protects against  LPS-induced  BV-2 activation  and MPTP- induced toxicity in SH-SY5Y neuronal cells. Journal of the Neurological Sciences, 333, e338. XXI World Congress of Neurology held at Reed Messe Wien GmbH Congress Center, Vienna, Austria, September 21-26, 2013.

Mesuagenin C mitigated  LPS-induced  neuroinflammation in BV-2 and  NG108-15 cells  through  pMTOR-PI3K-Akt  and CCL21  downregulation.  Journal of the  Neurological  Sciences, 333,  e349.  XXI World  Congress  of Neurology  held  at Reed Messe Wien GmbH Congress Center, Vienna, Austria, September 21-26, 2013. 2013.

Curcumenol from curcuma zedoaria suppresses neuroinflammation induced by LPS in BV-2 neuronal cell model. 18th Biological Sciences Graduate Congress, 06 Jan 2014 to 08 Jan 2014, Faculty of Science, University of Malaya.

Catechin  from  Loranthus parasiticus  (L.)  Merr attenuated  LPS- induced neuroinflammation in BV-2 cells. 38th The Malaysian Society for Biochemistry & Molecular Biology Annual Conference, 28 Aug 2013 to 29 Aug 2013, The Malaysian Society for Biochemistry & Molecular Biology (MSBMB), (National

(+)-Catechin Attenuates  NF-κB  Activation Through Regulation  of Akt, MAPK, and AMPK Signaling Pathways in LPS-Induced BV-2 Microglial Cells. 19th Biological  Sciences Graduate  Congress,  12 Dec  2014  to 14  Dec  2014, Department  of Biological Sciences in National University of Singapore (International).

Research Grants

IPPP – PostGraduate Research Fund (UM, 2012-2015). Molecular Mechanisms Of NF-κβ-CCL21 Regulation By Mesuagenin C-Induced Neutritogenesis In NG108-15 Cells.

IPPP – PostGraduate Research Fund (UM, 2011-2013). Studies on the Aquilaria sp. (Agarwood) and its chemical constituents on age-related disease and its possible neuroprotective mechanisms on human neuroblastoma (SH-SY5Y)

Fundamental Research Grant Scheme (FRGS): Investigation On CCL21-NF-κB Regulation By Catechin From Loranthus Parasiticus in In Vitro And In Vivo Alzheimer's Models. This is an award grant as for best FRGS Project 2014. Responsible for the write-up of this grant, the management of the grant and project execution.

High Impact Research (HIR) Project: Novel NF-κB - Chemokines Regulatory Mechanisms By Selected Phytochemicals on Neuronal Models.

UMRG Programme Grant - The effect of vermicomposting on the enhancement of selected medicinal plants and its therapeutic potential by nanotechnology (The evaluation of normal soil and vermicompost grown Clinacanthus nutans and Elephantopus scaber and their liposomal extracts against LPS-stimulated microglia cells).

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Awards

Best Thesis Award & Amersham Biosciences Prize by Malaysia Society of Biochemistry and Molecular Biology (MSBMB), Malaysia.

1st Prize Oral Presenter at InPRAS Conference UM 2014.

1st Prize Poster Presenter at World Congress on Healthy Ageing 2012

Best Poster Presentation, 39th Annual Malaysian Society for Biochemistry and Molecular Biology Conference, 2014, (INTERNATIONAL)

Silver Medal Winner for Poster Presentation (Biochemistry & Physiology), 19th Biological Sciences Graduate Congress, National University of Singapore, 2014 (International).

Silver Medal Winner for Poster Presentation (Biochemistry & Physiology), 18th Biological Sciences Graduate Congress, University of Malaya, 2014.

Poster Winner - 26th Inter Varsity Biochemistry Seminar, 26th Inter Varsity Biochemistry Seminar & Malaysian Society for Biochemistry and Molecular Biology, 2015, (NATIONAL)

Poster Winner (Two Undergraduate Students) - 24th Inter Varsity Biochemistry Seminar, 24th Inter Varsity Biochemistry Seminar,Malaysian Society for Biochemistry and Molecular Biology, 2013