Dr Syafiq Asnawi Zainal Abidin

Assistant Lecturer

Jeffrey Cheah School of Medicine & Health Sciences

Email: syafiq.asnawi@monash.edu
Tel: +603 5514 6282
Office: Room 4-4-48

ORCID ID: https://orcid.org/0000-0002-8249-7668

Profile Summary

Dr Syafiq Asnawi is an Assistant Lecturer at Jeffrey Cheah School of Medicine and Health Sciences. He holds a Bachelor of Biotechnology (Hons) degree majoring in Medical Biotechnology from Monash University, Clayton Campus, Australia in 2012. He obtained his PhD in 2018 in Biomedical Science from Monash University (Malaysia). His PhD research was focusing on the proteomic characterization and anti-cancer activities from the venom of Malaysian pit vipers on human colon cancer cells.

Throughout his studies, Dr Syafiq acquired the skills and was trained in various advanced proteomic techniques such as operating and maintaining mass spectrometry system (Agilent QTOF LCMS and Agilent QQQ LCMS) and data analysis using various advanced proteomic software such as PEAKS (Bioinformatics Solutions Inc., Canada) and Spectrum Mill (Agilent). He was previously appointed as the Principal Technical Officer at the LCMS Platform, JCSMHS, Monash University Malaysia.

Academic Qualifications

  • PhD (Biomedical Science), Monash University Malaysia
  • Bachelor of Biotechnology (Hons) majoring in Medical Biotechnology, Monash University, Clayton Campus, Australia
  • South Australian Certificate of Education (SACE), International Education Centre (INTEC), UiTM Shah Alam

Involvement in Organizations

  • Malaysian Society on Toxinology (MST) - Member
  • Pan ASEAN Anti-venom Research Group (PAAV) - Member
  • Monash University Malaysia, Monash Venom Group - Group Coordinator
  • Monash University Malaysia, Neuropharmacology Research Strength (Research Member - Neuroproteomics)
  • Monash University Malaysia, Microbiome and Bioresource Research Strength (Research Member - Proteomics)

Research & Education

Research Interests

  • Venomics (Snake venom) and Anti-venomics
  • Biomedical Science
  • Proteomics

Recent Publications

Rowshan Ara Islam, Hamed Al-Busaidi, Rahela Zaman, Syafiq Asnawi Zainal Abidin, Iekhsan Othman, Ezharul Hoque Chowdhury (2020), “Carbonate apatite and hydroxyapatite formulated with minimal ingredients to deliver siRNA into breast cancer cells in vitro and in vivo”, Journal of Functional Biomaterials, https://doi.org/10.3390/jfb11030063. (IF N/A, Q2)

Kha Wai Hon, Syafiq Asnawi Zainal Abidin, Iekhsan Othman, Rakesh Naidu (2020), “Insights into the role of microRNAs in colorectal cancer (CRC) metabolism”, Cancers (IF:6.162, Q1), 12, 9, 2462, https://doi.org/10.3390/cancers12092462

Hammad Saleem, Thet Thet Htar, Rakesh Naidu, Gokhan Zengin, Marcello Locatelli, Angela Tartaglia, Syafiq Asnawi Zainal Abidin, Nafees Ahemad (2020), “Phytochemical composition and enzyme inhibition studies of aerial and stem parts of Buxus papillosa C.K. Schneid”, Processes, https://doi.org/10.3390/pr8070757. (IF 2.753, Q2)

Thaarvena Retinasamy, Mohd Farooq Shaikh, Yatinesh Kumari, Syafiq Asnawi Zainal Abidin, Iekhsan Othman (2020), “Orthosiphon stamineus Extract Reverses Streptozotocin-induced Alzheimer’s Disease Like Condition in A Rat Model”, Biomedicines, https://doi.org/10.3390/biomedicines8050104 (IF 4.717, Q1)

Sultana Mehbuba Hossain, Syafiq Asnawi Zainal Abidin, Md. Ezharul Hoque Chowdhury (2020), “Krebs Cycle Intermediate-modified Carbonate Apatite Nanoparticles drastically reduce mouse tumor burden and toxicity by restricting broad tissue-distribution of anti-cancer drugs”, Cancers, 12, 1, https://doi.org/10.3390/cancers12010161. (IF: 6.162, Q1)

Yam Nath Paudel, Yatinesh Kumari, Syafiq Asnawi Zainal Abidin, Iekhsan Othman, Mohd. Farooq Shaikh (2020), “Pilocarpine Induced Behavioral and Biochemical Alterations in Chronic Seizure-Like Condition in Adult Zebrafish”, International Journal of Molecular Sciences, 21, 7, 2492, https://doi.org/10.3390/ijms21072492. (IF 4.556, Q1)

Sheikh Tanzina Haque, Md. Emranul Karim, Syafiq Asnawi Zainal Abidin, Iekhsan Othman, Mark Banaszak Holl, Ezharul Hoque Chowdhury (2020), “Fe/Mg-Modified Carbonate Apatite with Uniform Particle Size and Unique Transport Protein-Related Protein Corona Efficiently Delivers Doxorubicin into Breast Cancer Cells”, Nanomaterials, https://doi.org/10.3390/nano10050834 (IF 4.324, Q1)

Hammad Saleem, Umair Khurshid, Saeed Ahmad, Gokhan Zengin, Marcello Locatelli, Hafiz Awaiz, Nawaz, Mohamad Fawzi Mahomoodally, Syafiq Asnawi Zainal Abidin, Nafees Ahemad (2019), “Phytochemical composition and in vitro pharmacological investigations of Neurada procumbes L. (Neuradaceae): A multidirectional approach for industrial products”, Industrial Crops and Products, https://doi.org/10.1016/j.indcrop.2019.111861 (IF: 4.191, Q1).

Hammad Saleem, Gokhan Zengin, Marcello Locatelli, Irshad Ahmad, Saharish Khaliq, Fawzi Mahomoodally, Riaz Hussain, Kannan Rengasamy, Adriano Mollica, Syafiq Asnawi Zainal Abidin, Nafees Ahemad (2019), “Pharmacological, phytochemical and in-vivo toxicological perspectives of a Xero-halophyte medicinal plant: Zaleya Pentandra (L.) Jeffrey”, Food and Chemical Toxicology, https://doi.org/10.1016/j.fct.2019.05.043 (IF: 3.775, Q1).

Uday Abdullah, Hishamshah Ibrahim, Noraesah Mahmud, Mohamad Zaki Salleh, The Lay Kek, Mohd Nur Fakhruzzaman Noorizhab, Iekhsan Othman, Syafiq Asnawi Zainal Abidin, Haitham Muhammed Jassim, Bin Zilfalil, Prapin Wilairat, Suthat Fucharoen (2019), “Quadrupole-Time-of-Flight Mass Spectrometric Identification of Hemoglobin Subunits α, β, γ and δ in Unknown Peaks of High Performance Liquid Chromatography of Hemoglobin in β-Thalassemias”, Hemoglobin, https://doi.org/10.1080/03630269.2019.1632893

Mei Yeng Yew, Rhun Yian Koh, Soi Moi Chye, Iekhsan Othman, Syafiq Asnawi Zainal Abidin, Khuen Yen Ng (2019), “Neurotrophic properties and de novo peptide sequencing of edible bird’s nest extracts”, Food Bioscience, doi: https://doi.org/10.1016/j.fbio.2019.100466.

Janeyuth Chaisakul, Muhamad Rusdi Ahmad Rusmili, Iekhsan Othman, Syafiq Asnawi Zainal Abidin, Fathin Yusof, Kavi Ratanabanangkoon, Lawan Chanhome, Wayne C. Hodgson (2019), “Geographical variation of Malayan krait (Bungarus candidus) venoms”, PLOS ONE

Hamed Al-Busaidi, Md. Emranul Karim, Syafiq Asnawi Zainal Abidin, Ezharul Hoque Chowdhury (2019), “Magnesium fluoride forms unique protein corona for efficient delivery of doxorubicin into breast cancer cells”, Toxics, 7(1), 10, doi: https://doi.org/10.3390/toxics7010010.

Hammad Saleem, Gokhan Zengin, Kashif-ur Rehman Khan, Irshad Ahmad, Muhammad Waqas, Fawzi M. Mahomoodally, Kannan R.R. Rengasamy, Noorazwani Zainol, Syafiq Asnawi Zainal Abidin, Nafees Ahemad (2019), “New insights into the phytochemical composition, enzyme inhibition and antioxidant properties of desert cotton (Aerva javanica (Bum.f) Shult. – Amaranthaceae”), Natural Product Research, doi: https://doi.org/10.1080/14786419.2019.1587427

Syafiq Asnawi Zainal Abidin, Yee Qian Lee, Iekhsan Othman, Rakesh Naidu (2019), “Malaysian Cobra Venom: A Potential Source for Anti-cancer Therapeutic Agents”, Toxins, 11(2), 75, doi: https://doi.org/10.3390/toxins11020075

Muhammad S. Hishamuddin, Shiou Yih Lee, Nurulfiza Mat Isa, Dhilia U Lamasudin, Syafiq Asnawi Zainal Abidin, Rozi Mohamed (2019), “Time-based LC-MS/MS Analysis Provides Insights into Early Responses to Mechanical Wounding, A Major Trigger to Agarwood Formation in Aquilaria malaccensis Lam”, RSC Advances, https://doi.org/10.1039/c8ra10616a.

Hammad Saleem, Gokhan Zengin, Marcello Locatelli, Adriano Mollica, Irshad Ahmad, Fawzi M. Mahomoodally, Syafiq Asnawi Zainal Abidin, Nafees Ahemad (2019), “In vitro biological propensities and chemical profiling of Euphorbia milii Des Moul (Euphorbiaceae): A novel source for bioactive agents”, Industrial Crops and Products, https://doi.org/10.1016/j.indcrop.2018.12.062.

Hammad Saleem; Thet Thet; Rakesh Naidu; G, Zengin; Syafiq Asnawi Zainal Abidin; Nafees Ahemad (2018), “Insight into the chemical, biological and toxicological properties of Anagallis arvensis L. and Bougainvillea glabra (Choisy.): A comprehensive approach”, Food and Chemical Toxicology (IF: 3.775, Q1), submitted 9th October 2018.

Hammad Saleem, Irshad Ahmad, Gokhan Zengin, Fawzi M. Mahomoodally, Hafiz Muhammad Ahsan, Syafiq Asnawi Zainal Abidin, Nafees Ahemad (2018), “Comparative secondary metabolites profiling and biological activities of aerial, stem and root parts of Salvadora oleoides Decne (Salvadoraceae), Natural Product Research, https://doi.org/10.1080/14786419.2018.1564299.

Ashish Gautham; Yam Paudel; Syafiq Asnawi Zainal Abidin, Uma Bhandari (2018), “Guggulesterone, a Farnesoid X Receptor antagonist lowers plasma Trimetyhlamine-N-oxide levels: An evidence from in vitro and in vivo studies”, Human and Experimental Toxicology, https://doi.org/10.1177/0960327118817862.

Syafiq Asnawi Zainal Abidin; Pathmanathan Rajadurai; Md. Ezharul Hoque Chowdhury; Muhammad Rusdi Ahmad Rusmili; Iekhsan Othman; Rakesh Naidu (2018), “Cytotoxic, anti-proliferative and apoptosis inducing activity of L-amino acid oxidase (LAAO) from Malaysian Calloselasma rhodostoma on human colon cancer cells”, Basic & Clinical Pharmacology and Toxicology, https://doi.org/10.1111/bcpt.13060.

Syafiq Asnawi Zainal Abidin; Pathmanathan Rajadurai; Md. Ezharul Hoque Chowdhury; Iekhsan Othman; Rakesh Naidu. (2018), “Cytotoxic, Anti-proliferative and Apoptosis Activity of L-Amino Acid Oxidase from Malaysian Cryptelytrops purpureomaculatus (CP-LAAO) Venom on Human Colon Cancer Cells”, Molecules 2018, 23(6), 1388; https://doi.org/10.3390/molecules23061388.

Syafiq Asnawi Zainal Abidin; Pathmanathan Rajadurai; Md. Ezharul Hoque Chowdhury; Muhammad Rusdi Ahmad Rusmili; Iekhsan Othman; Rakesh Naidu. (2016), “Proteomic Characterization and Comparison of Malaysian Tropidolaemus wagleri and Cryptelytrops purpureomaculatus Venom Using Shotgun-Proteomics”, Toxins, 8(10), 299, DOI: https://doi.org/10.3390/toxins8100299.

Research Grants

Principal Investigator

  • Funder: Monash Tropical Medicine and Biology Multidisciplinary Research Platform (TMB), Monash University Malaysia, Malaysia
    Year: 2020
    Amount: RM10 000
    Co-Investigators: Prof. Iekhsan Othman (JCSMHS, MUM); Assoc. Prof. Kumaran Narayanan. (MUM-Science)
    Project Title: Proteomic characterization of the venom from Malaysian venomous jellyfish, Carybdea alata
  • Funder: Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Malaysia
    Year: 2020
    Amount: RM 10,000
    Co-Investigators: Prof. Iekhsan Othman (JCSMHS, MUM); Assoc. Prof. Rakesh Naidu (JCSMHS, MUM);
    Project Title: Identification of antigenic proteins from the venom of Malaysian venomous snakes using tandem mass spectrometry
  • Funder: Wellcome Trust UK – Royal Society of Tropical Medicine and Hygiene (RSTMH) (Research grant for snakebite envenoming project), United Kingdom
    Year: 2020
    Amount: £ 5000 (Approx. RM 26,305)
    Project Title: Identification of antigenic proteins from the venom of Malaysian venomous snakes using tandem mass spectrometry
  • Funder: Global Asia in the 21st Century (GA21) Platform, Monash University Malaysia, Malaysia
    Year: 2018
    Amount: RM 10,000
    Co-Investigators: Prof. Iekhsan Othman (JCSMHS, MUM); Dr. Mohmad Farooq Shaikh (JCSMHS, MUM)
    Project Title: Investigation of the Anticonvulsive Effect of the Venom from Malaysian Pit Vipers; Calloselasma rhodostoma (Malayan pit viper) and Cryptelytrops purpureomaculatus (Mangrove pit viper) using in vivo Zebrafish Model.

Associate/Co-investigator

  • Funder: JCSMHS-BRIMS Collaboration Grant, Monash University Malaysia, Malaysia
    Year: 2020
    Principal Investigator: Prof. Ammu Radakrishnan. (JCSMHS, MUM)
    Amount: RM20,000
    Project Title: Discovery of biomarkers of following supplementation of tocotrienols in two human glioblastoma cells using quantitative proteomics profiling strategies
  • Funder: Wellcome Trust, Wellcome Trust Foundation UK, United Kingdom
    Year: 2019
    Principal Investigator: Prof. Iekhsan Othman (JCSMHS, MUM)
    Amount: £ 139,997
    Project Title: Antivenom Market Study: Burden of Snakebite and Its Situation Analysis in ASEAN

Research Supervision

Hina Rasheed (Doctor of Philosophy, Pharmacy)
Thesis title: “Elucidation of neuroprotective effect of zinc oxide nano-particles in Parkinson’s disease model”
2020 - ongoing
Co-supervisor with Dr. Tang Kim San

Nurul Atiqah Zulazmi (Doctor of Philosophy, BMedSci)
Thesis title: “Impact of traumatic brain injury on epileptogenesis and neuropsychiatric comorbidities in a zebrafish model”
2020 - ongoing
Co-supervisor with Dr Mohmmad Farooq Shaikh and Dr Idrish Ali

Chen Ken Fong (Doctor of Philosophy, Pharmacy)
Thesis title: “Elucidating the infective pathway of the Macrobrachium rosenbergii nodavirus”
2020 - ongoing
Co-supervisor with Dr. Ronald Lee Fook Seng (Main Supervisor), Prof. Dr. Ong Lin Kooi, and Tan Wen Siang

Dominic Hon Kha Wai (Doctor of Philosophy, BMedSci)
Thesis title: “Molecular mechanisms of a selected diarylpentanoid treated on human colon cancer cells”
2019 - ongoing
Co-supervisor with Assoc. Prof. Rakesh Naidu (Main Supervisor)

Eugene Yew Soon (BSci Honours)
Thesis title: "Snake venom protein as a therapeutic strategy for epilepsy and memory"
2019-2020
Co-supervisor with Dr. Mohmad Farooq Shaikh and Dr. Michelle Yap (School of Science)

Rena Yeo Li Kheng (Young Scholars Program 2019)
Project Title: "Purification of L-amino acid oxidase from the venom of Malaysian Calloselasma rhodostoma(Malayan pit viper)"
2019
Co-supervisor with Prof. Iekhsan Othman and Assoc. Prof. Rakesh Naidu

Ivan Ruben V. Visagaran (Young Scholars Program 2019)
Project Title: "Purification of L-amino acid oxidase from the venom of Malaysian Calloselasma rhodostoma (Malayan pit viper)"
Co-supervisor with Prof. Iekhsan Othman and Assoc. Prof. Rakesh Naidu

William Wong Lik Loong (MED5101 Scholarly Intensive Placement)
Project Title: "Intra-species and inter-species cross reactivity studies of various anti-venoms against Malaysian venomous snakes"
Co-supervisor with Prof. Iekhsan Othman and Assoc. Prof. Rakesh Naidu

Ong Von He (BMedSci Honours)
Thesis title: "Venomics and anti-venomics of the venom from Malaysian venomous terrestrial snakes using LCMSMS" 2018-2019
Co-supervisor with Prof. Iekhsan Othman and Assoc. Prof. Rakesh Naidu

Achievements

Oral Presentation:

Syafiq Asnawi Zainal Abidin, Pathmanathan Rajadurai, Md. Ezharul Hoque Chowdhury, Muhamad Rusdi Ahmad Rusmili, Iekhsan Othman, Rakesh Naidu. (2016). “Bioassay Guided Purification of Anti-cancer Proteins from C. rhodostoma using LCMSMS”. International Society of Toxinology (IST) Pan American Congress, Miami Beach, Florida, U.S.A.

Poster Presentation:

Syafiq Asnawi Zainal Abidin, Pathmanathan Rajadurai, Md. Ezharul Hoque Chowdhury, Iekhsan Othman, Rakesh Naidu (2015). “Proteomic characterization of Malaysian pit vipers; Calloselasma rhodostoma and Cryptelytrops purpureomaculatus crude venoms via shotgun-proteomic tandem mass spectrometry (LC-MS/MS) approach”. Mass Spec 2015: New Horizons in MS Hyphenated Techniques and Analyses, Biopolis, Singapore.

Syafiq Asnawi Zainal Abidin, Pathmanathan Rajadurai, Iekhsan Othman, Rakesh Naidu (2018). “Rhodocytin: a non-cytotoxic protein from the venom of Malayan pit viper (Calloselasma rhodostoma) with potential anti-migratory activity on human colon cancer cells” Accepted for the 8th Regional Conference on Molecular Medicine (RCMM) in Conjunction With 4th National Conference for Cancer Research 2018, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. (Poster presentation abstract).

Abstracts:

Nur Atiqah Haizum Abdullah, Syafiq Asnawi Zainal Abidin, Muhamad Rusdi Ahmad Rusmili, Mohd Farooq Shaikh, Iekhsan Othman (2020). “Proteomic characterization of black Malaysian spitting cobra, Naja sumatrana venom using electrospray ionization liquid chromatography-mass spectrometry (ESI-LCMSMS) and its neurotoxicity activity”. Accepted and Presented for the Jeffrey Cheah School of Medicine and Health Sciences Research Symposium, Monash University Malaysia, Kuala Lumpur, Malaysia, 15th-16th January 2020

Iekhsan Othman, Syafiq Asnawi Zainal Abidin, Nur Atiqah Abdullah, Fathin Athirah Yusof, Mohmad Farooq Shaikh, Khaldun Ahmad Ismail, Muhamad Rusdi Ahmad Rusmili, Rakesh Naidu (2019). “Targeted proteomics for neurotoxic and anti-cancer proteins from snake venoms: a LCMSMS and bioassay workflows”.Presented for the Advanced Omics Technologies & Approaches, Mumbai, India, 17-18th January 2019.

Abdinasir Yusuf Osman, Suratan Kamarudin, Syafiq Asnawi Zainal Abidin, Iekhsan Othman (2018). “Prevalence of residual antibiotics in the surface water and from poultry farms in Kelantan state, Malaysia”. University Malaysia Kelantan, December 2018.

Iekhsan Othman, Syafiq Asnawi Zainal Abidin, Fathin Athirah Yusof, Khaldun Ahmad Ismail, and Rakesh Naidu (2018). “Applications of Mass Spectrometry in Identification of Potential Anticancer Proteins from Snake Venoms”. Presented for the ASEAN Marine Animals and Snake Envenoming (Poisoning) Management 2018, The 5th International Symposium, Hotel Neo Plus Awana, Yogyakarta, Indonesia. 23-26th October 2018 (Keynote Address).

Iekhsan Othman, Syafiq Asnawi Zainal Abidin, Nur Atiqah Abdullah, Mohd Farooq Shaikh, Khaldun Ahmad Ismail, Muhamad Rusdi Ahmad Rusmili, and Rakesh Naidu (2018). “Marine animals and snakes: their venoms and poisons for therapeutics”. Presented for the ASEAN Marine Animals and Snake Envenoming (Poisoning) Management 2018, The 5th International Symposium, Hotel Neo Plus Awana, Yogyakarta, Indonesia. 23-26th October 2018 (Oral presentation abstract).

Syafiq Asnawi Zainal Abidin, Pathmanathan Rajadurai, Iekhsan Othman, Rakesh Naidu (2018). “Rhodocytin: a non-cytotoxic protein from the venom of Malayan pit viper (Calloselasma rhodostoma) with potential anti-migratory activity on human colon cancer cells” Accepted for the 8th Regional Conference on Molecular Medicine (RCMM) in Conjunction With 4th National Conference for Cancer Research 2018, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. (Poster presentation abstract).

Syafiq Asnawi Zainal Abidin, Pathmanathan Rajadurai, Md. Ezharul Hoque Chowdhury, Muhamad Rusdi Ahmad Rusmili, Iekhsan Othman, Rakesh Naidu. (2016). “Bioassay Guided Purification of Anti-cancer Proteins from C. rhodostoma using LCMSMS”. International Society of Toxinology (IST) Pan American Congress, Miami Beach, Florida, U.S.A. (Oral presentation abstract).

Syafiq Asnawi Zainal Abidin, Pathmanathan Rajadurai, Md. Ezharul Hoque Chowdhury, Iekhsan Othman, Rakesh Naidu (2015). “Proteomic characterization of Malaysian pit vipers; Calloselasma rhodostoma and Cryptelytrops purpureomaculatus crude venoms via shotgun-proteomic tandem mass spectrometry (LC-MS/MS) approach”. Mass Spec 2015: New Horizons in MS Hyphenated Techniques and Analyses, Biopolis, Singapore. (Poster presentation abstract).

Other Achievements:

Winner – Three Minutes Thesis (3MT) Competition Campus Final (Monash University Malaysia)(2016)

Finalist – 3MT Competition Faculty Level (Monash University Australia) (2016)