Dr. Ronald Lee Fook Seng

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Lecturer  
School of Pharmacy

Ronald.Lee@monash.edu
+603 5515 9755
Room  2-5-49

Dr. Ronald is a Lecturer at Monash University Malaysia. His research is mainly in the study of proteins and their involvement in drug action and disease states. Dr. Ronald received his Bachelor of Science (Pharmacy) from the National University of Singapore (NUS) in 2010. He subsequently underwent pre-registration training at Changi General Hospital and obtained registration as a Pharmacist in Singapore. Following this, he worked for a year as a research assistant in the Department of Pharmacy, NUS working on development of zebrafish as a model to study the metabolism of xenobiotics. After that, he did his PhD in the École polytechnique fédérale de Lausanne on the application of mass spectrometry (MS) to study the mechanism of transition metal based anti-cancer compounds. Here, his work involved the application of MS techniques such as Nano-SIMS, top-down MS and protein expression profiling strategies to study various aspects of drug action such as cellular distribution, protein targets and also specific binding sites. Upon completion of his PhD in 2017, he worked as a post-doctoral fellow in the National Cancer Center in Singapore, setting up a single cell genomics core facility before his transition to Monash University Malaysia.

Qualifications

- Doctor of Philosophy in Chemistry and Chemical Engineering, École polytechnique fédérale de Lausanne, 2017
- Bachelor of Science (Pharmacy), National University of Singapore, 2010

Expertise

1. Mass Spectrometry
2. Analytical Chemistry
3. Biochemistry

Research Experience and Employment History

2019  -  Present  Lecturer, Monash University Malaysia
2017-2018   Research Fellow, National Cancer Center Singapore
2012-2017   Doctoral Assistant, École polytechnique fédérale de Lausanne, Switzerland
2011-2012   Research Assistant, Department of Pharmacy, National University of Singapore
2010-2011   Pre-registration Pharmacist, Changi General Hospital, Singapore

Professional Affiliations

Registered Pharmacist, since 2011
Pharmacy Board of Singapore

Research Interest

I plan to apply my expertise in mass spectrometry to analytical characterization of protein based therapeutics and identification of protein receptors. I am open to discussions for any collaborative projects where I can provide value from my field of expertise.

Scholarly Journals (Refereed Only)

Lee, R. F. S., Menin, L., Patiny, L., Ortiz, D. & Dyson, P. J. Versatile tool for the analysis of metal–protein interactions reveals the promiscuity of metallodrug–protein interactions. Analytical Chemistry.2017, 89, 11985–11989.  URL: https://pubs.acs.org/doi/10.1021/acs.analchem.7b02211

Lee, R. F. S., Escrig, S., Maclachlan, C., Knott, G.W., Meibom, A., Sava, G., and Dyson, P.J. The differential distribution of RAPTA-T in non-invasive and invasive breast cancer cells correlates with its anti-invasive and anti-metastatic effects. International Journal of Molecular Sciences. 2017, 18, 1869. URL: http://www.mdpi.com/1422-0067/18/9/1869

Lee, R.F.S., Riedel, T., Escrig, S., Maclachlan, C., Knott, G.W., Davey, C.A., Johnsson, K., Meibom, A., and Dyson, P.J. Differences in cisplatin distribution in sensitive and resistant ovarian cancer cells: a TEM/NanoSIMS study. Metallomics. 2017, 9, 1413–1420.
URL: http://pubs.rsc.org/en/Content/ArticleLanding/2017/MT/C7MT00153C#!divAbstract

Lee, R.F.S., Chernobrovkin, A., Rutishauser, D., Allardyce, C.S., Hacker, D., Johnsson, K., Zubarev, R.A., and Dyson, P.J. Proteomics to determine metallodrug targets and optimal drug combinations. Scientific Reports. 2017, 7, 1590. URL:  https://www.nature.com/articles/s41598-017-01643-1

Temboot, P., Lee, R.F.S., Menin, L., Patiny, L., Dyson, P.J., and Ratanaphan, A. Biochemical and biophysical characterization of ruthenation of BRCA1 RING protein by RAPTA complexes and its E3 ubiquitin ligase activity. Biochemical and Biophysical Research Communications. 2017, 488, 355-361. URL: http://www.sciencedirect.com/science/article/pii/S0006291X17309142

Lee, R.F.S.,* Theiner, S.,* Meibom, A., Koellensperger, G., Keppler, K.K., Dyson, P.J. Application of imaging mass spectrometry approaches to facilitate metal-based anticancer drug research. Metallomics. 2017, 9, 365–381. (*Co-first authors).
URL: http://pubs.rsc.org/en/content/articlelanding/2017/mt/c6mt00231e

Lee, R.F.S., Escrig, S., Croisier, M., Clerc-Rosset, S., Knott, G.W., Meibom, A., Davey, C.A., Johnsson, K., and Dyson, P.J. NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro. Chemical Communications. 2015, 51, 16486–16489. URL: http://pubs.rsc.org/en/content/articlelanding/2015/cc/c5cc06983a

Chaudhury, A., Das, S., Lee, R.F.S., Tan, K.-B., Ng, W.-K., Tan, R.B.H., and Chiu, G.N.C. Lyophilization of cholesterol-free PEGylated liposomes and its impact on drug loading by passive equilibration. International Journal of Pharmaceutics. 2012, 430, 167–175.
URL: http://linkinghub.elsevier.com/retrieve/pii/S0378517312003821

Conferences

Lee R.F.S., Johnsson, K., and Dyson, P.J.  Understanding the cellular distribution and protein targets of a ruthenium(II) anti-cancer compound, RAPTA-T via mass spectrometry.  Swiss Chemical Society Fall Meeting; 2016 Sept; Zurich, Switzerland.

Lee, R.F.S., Meibom, A., Escrig, S., Knott, G., Croisier, M., Clerc-Rosset, S., Johnsson, K., and Dyson, P.J. Nano-SIMS to study the distribution of metal based anti-cancer compounds in vitro.  International Mass Spectrometry Conference; 2016 Aug, Toronto; Canada.

Lee, R.F.S., Menin, L., Patiny, L., Dyson, P.J. Investigating the binding sites of RAPTA-C and RAPTA-EA to a 50 amino acid peptide using ETD fragmentation and ChemInfo algorhythms.  Swiss Chemical Society Fall Meeting; 2015 Sept; Lausanne, Switzerland.

Lee, R.F.S., Johnsson, K., and Dyson, P.J. Exploring cytotoxicity of O6-Benzylguanine tethered platinum and ruthenium anti-cancer complexes. XXIII International Symposium on Medicinal Chemistry; 2014 Sept; Lisbon, Portugal.

Chng, H.T., Lee, R.F.S, Lam, S.H., Gong, Z., Ho, H.K., Chan, E.C.Y. Zebrafish: an in vivo high-throughput screening model for CYP3A4 induction and inhibition.  12th European Regional International Society for the Study of Xenobiotics meeting; 2012 June; Noordwjik aan Zee, the Netherlands.

Wee, I.W.J., Lee, R.F.S. Evaluation of the RIFLE and AKIN criteria for detection of acute kidney injury in critically ill patients.  21st Singapore Pharmacy Congress; 2011 July; Singapore.

Chaudhury, A., Lee, R.F.S., and Chiu. G.N.C. Freeze-drying of cholesterol-free sterically stabilized liposomeand subsequent drug loading.  FIP Pharmaceutical Sciences World Congress in association with the AAPS Annual Meeting and Exposition & Pharmaceutical Sciences World Congress; 2010 Nov; New Orleans, LA, USA.

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