Dr. Ronald Lee Fook Seng

.

Lecturer  
School of Pharmacy

Ronald.Lee@monash.edu
+603 5515 9755
Room  2-5-49

Dr. Ronald is a Lecturer at Monash University Malaysia. His research is mainly in the study of proteins and their involvement in drug action and disease states. Dr. Ronald received his Bachelor of Science (Pharmacy) from the National University of Singapore (NUS) in 2010. He subsequently underwent pre-registration training at Changi General Hospital and obtained registration as a Pharmacist in Singapore. Following this, he worked for a year as a research assistant in the Department of Pharmacy, NUS working on development of zebrafish as a model to study the metabolism of xenobiotics. After that, he did his PhD in the École polytechnique fédérale de Lausanne on the application of mass spectrometry (MS) to study the mechanism of transition metal based anti-cancer compounds. Here, his work involved the application of MS techniques such as Nano-SIMS, top-down MS and protein expression profiling strategies to study various aspects of drug action such as cellular distribution, protein targets and also specific binding sites. Upon completion of his PhD in 2017, he worked as a post-doctoral fellow in the National Cancer Center in Singapore, setting up a single cell genomics core facility before his transition to Monash University Malaysia.

Qualifications

- Doctor of Philosophy in Chemistry and Chemical Engineering, École Polytechnique Fédérale de Lausanne, 2017

- Bachelor of Science (Pharmacy), National University of Singapore, 2010

Expertise

- Mass Spectrometry

- Analytical Chemistry

- Biochemistry

Employment History

2019 - Present: Lecturer, School of Pharmacy, Monash University Malaysia

2017 - 2018: Research Fellow, National Cancer Center Singapore

2012 - 2017: Doctoral Assistant, École polytechnique fédérale de Lausanne, Switzerland

2011 - 2012: Research Assistant, Department of Pharmacy, National University of Singapore

2010 - 2011: Pre-registration Pharmacist, Changi General Hospital, Singapore

Professional Affiliation

2019

  • Registered Pharmacist, Malaysia Pharmacy Board

2011

  • Registered Pharmacist, Singapore Pharmacy Board

Dr. Ronald applies chemical, biochemical and analytical approaches to study critical problems in the field of food security and human health. He is currently engaged in projects involving studying the mechanisms of viral pathogens affecting aquaculture, natural product formulations and application of ionic liquids on food and natural product processing.

Teaching & Education Research

- PHR1022 (How Medicines Work II)

- PHR2021 (How Medicines Work III)

- PHR2042 (Comprehensive Care III - Endocrinology and Renal)

Scholarly Journals (Refereed Only)

2020

Maroof, K*., Lee, R. F. S., Siow, L.F., Gan, S.H. Microencapsulation of propolis by spray drying: A review. Drying Technology. 2020. In-press.

2017

Lee, R. F. S., Menin, L., Patiny, L., Ortiz, D. & Dyson, P. J*. Versatile tool for the analysis of metal–protein interactions reveals the promiscuity of metallodrug–protein interactions. Analytical Chemistry.2017, 89, 11985–11989.

Lee, R. F. S., Escrig, S., Maclachlan, C., Knott, G.W., Meibom, A., Sava, G., and Dyson, P.J*. The differential distribution of RAPTA-T in non-invasive and invasive breast cancer cells correlates with its anti-invasive and anti-metastatic effects. International Journal of Molecular Sciences. 2017, 18, 1869.

Lee, R.F.S., Riedel, T., Escrig, S., Maclachlan, C., Knott, G.W., Davey, C.A., Johnsson, K., Meibom, A., and Dyson, P.J*. Differences in cisplatin distribution in sensitive and resistant ovarian cancer cells: a TEM/NanoSIMS study. Metallomics. 2017, 9, 1413–1420.

Lee, R.F.S., Chernobrovkin, A., Rutishauser, D., Allardyce, C.S., Hacker, D., Johnsson, K., Zubarev, R.A., and Dyson, P.J*. Proteomics to determine metallodrug targets and optimal drug combinations. Scientific Reports. 2017, 7, 1590.

Temboot, P., Lee, R.F.S., Menin, L., Patiny, L., Dyson, P.J., and Ratanaphan, A. Biochemical and biophysical characterization of ruthenation of BRCA1 RING protein by RAPTA complexes and its E3 ubiquitin ligase activity. Biochemical and Biophysical Research Communications. 2017, 488, 355-361.

Lee, R.F.S., &Theiner, S., Meibom, A., Koellensperger, G., Keppler, K.K., Dyson, P.J*. Application of imaging mass spectrometry approaches to facilitate metal-based anticancer drug research. Metallomics. 2017, 9, 365–381.

2015

Lee, R.F.S., Escrig, S., Croisier, M., Clerc-Rosset, S., Knott, G.W., Meibom, A., Davey, C.A., Johnsson, K., and Dyson, P.J*. NanoSIMS analysis of an isotopically labelled organometallic ruthenium(II) drug to probe its distribution and state in vitro. Chemical Communications. 2015, 51, 16486–16489.

Conferences

Lee R.F.S., Johnsson, K., and Dyson, P.J.  Understanding the cellular distribution and protein targets of a ruthenium(II) anti-cancer compound, RAPTA-T via mass spectrometry.  Swiss Chemical Society Fall Meeting; 2016 Sept; Zurich, Switzerland.

Lee, R.F.S., Meibom, A., Escrig, S., Knott, G., Croisier, M., Clerc-Rosset, S., Johnsson, K., and Dyson, P.J. Nano-SIMS to study the distribution of metal based anti-cancer compounds in vitro.  International Mass Spectrometry Conference; 2016 Aug, Toronto; Canada.

Lee, R.F.S., Menin, L., Patiny, L., Dyson, P.J. Investigating the binding sites of RAPTA-C and RAPTA-EA to a 50 amino acid peptide using ETD fragmentation and ChemInfo algorhythms.  Swiss Chemical Society Fall Meeting; 2015 Sept; Lausanne, Switzerland.

Lee, R.F.S., Johnsson, K., and Dyson, P.J. Exploring cytotoxicity of O6-Benzylguanine tethered platinum and ruthenium anti-cancer complexes. XXIII International Symposium on Medicinal Chemistry; 2014 Sept; Lisbon, Portugal.

Chng, H.T., Lee, R.F.S, Lam, S.H., Gong, Z., Ho, H.K., Chan, E.C.Y. Zebrafish: an in vivo high-throughput screening model for CYP3A4 induction and inhibition.  12th European Regional International Society for the Study of Xenobiotics meeting; 2012 June; Noordwjik aan Zee, the Netherlands.

Wee, I.W.J., Lee, R.F.S. Evaluation of the RIFLE and AKIN criteria for detection of acute kidney injury in critically ill patients.  21st Singapore Pharmacy Congress; 2011 July; Singapore.

Chaudhury, A., Lee, R.F.S., and Chiu. G.N.C. Freeze-drying of cholesterol-free sterically stabilized liposome and subsequent drug loading. FIP Pharmaceutical Sciences World Congress in association with the AAPS Annual Meeting and Exposition & Pharmaceutical Sciences World Congress; 2010 Nov; New Orleans, LA, USA.

Research Grants (Present)

Type of Grant: Fundamental Research Grant Scheme [FRGS/1/2019/STG04/MUSM/03/2]
Title of Research: Understanding the infection mechanism of the Macrobrachium rosenbergii nodavirus
Main Investigator: Ronald Lee
Co-Investigator: Professor Tan Wen Siang, Professor Iekhsan Othman, Professor Tey Beng Ti
Date/Duration: 2019 - 2022
Amount: RM 165,000

Research Grants (Completed)

Type of Grant: Early Career Researcher Grant Scheme [ECRG-2019-07-PHA]
Title of Research: Biocompatible Ionic Liquids as Extraction media for Removal of Free Fatty Acids from Palm Oil
Main Investigator: Ronald Lee
Co-Investigator: Khaw Kooi Yeong
Date/Duration: 2019 - 2020
Amount: RM 20,000


Type of Grant: Pharmacy SEED Grant [SG-1-19]
Title of Research: “Mapping student perspectives of an extensively flipped learning based Pharmacy degree in Malaysia”
Main Investigator: Wong Wei Jin & Ronald Lee
Date/Duration: 2019-2020
Amount: RM 20,000


Type of Grant: Tropical Medicines and Biology Platform Grant
Title of Research: Mapping host-receptor interactions of the Macrobrachium rosenbergii nodavirus
Main Investigator: Ronald Lee
Co-Investigator: Professor Tan Wen Siang, Professor Iekhsan Othman
Date/Duration: 2019-2020
Amount: RM 8,000

Postgraduate (PhD)

Chen Ken Fong (PhD: 2020 - present )
Elucidating the mechanism of infection of the Macrobrachium rosenbergii nodavirus
- Main Supervisor

Kashif Maroof (PhD: 2019 - present)
Development of new, stable, alcohol-free and halal propolis liquid and solid formulations
- Co-supervisor

Undergraduate

Ang Yi Wen & Ng Joe Wen (2020)
The uncommon benefits of palm oil for human health
- Main Supervisor

Neo Wan Bin (2020)
Optimizing removal of free fatty acids from palm oil
- Main Supervisor

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