Drug Discovery

BRIMS has introduced an induced pluripotent stem (iPS) cell system to generate adult human cells an in vitro approach for drug discovery. iPS cells were discovered by Professor Yamanaka who was awarded the 2012 Nobel Prize. iPS cells can be transformed into any other cell type. The iPS cell system has created huge advances in drug discovery and cell therapy for individual patients as donor-free regenerative medicine.

SPF Animal Facility operated by BRIMS is Malaysia’s first Specific Pathogen Free (SPF) facility and home to approximately 1,000 Mice and Rats. We have SPF rats and C57BL6N mice with health certifications and two transgenic lines of Wister rats. This platform provides laboratory animal research services.

The fish facility holds wild-type zebrafish (Danio rerio) and tilapia (Oreochromis niloticus) and various genetically modified zebrafish strains introduced from several research institutions including Japan, Hong Kong, China, Singapore, Canada, Germany and the United States. Furthermore, we have the capability to create transgenic and mutant zebrafish lines using CRISPR/Cas9 technique. We have also established behavior systems for fish models to study neurodegenerative disease, social stress, addiction, neuropsychiatric disorders and cognition.

Equipment available

iPS cell facility
Biosafety cabinets (Class II)
CO2 incubator with  O2 regulation
Single cuvette-based transfection system

SPF animal facility
Anesthetic machine
Microinjection system
Fume hood
Rodent behaviour analysis system
Rodent holding facility

Fish Facility
Fish holding facility (tilapia and zebrafish)
Photoperiod controlled fish holding rack
ZebTEC Stand-Alone Rack with Active Blue technology (self-cleaning system with water quality monitoring unit)
Fish behaviour analysis system
Microinjection system:  Eppendorf Femtojet

iPS Cell Facility

Equipment Model Identification Short Description of Capabilities Quantity
  Biosafety cabinet (Thermo Scientific™ 1300 Series Class II, Type A2)

It delivers superior protection with proprietary airflow design, exceptional ergonomics for a safe and comfortable environment for cell culture study. It is suitable ffor humaniPS cell, stem cell and primary cell culture.

2
  CO2 incubator with O2 regulation (Eppendorf New Brunswick Galaxy CO2 Incubator 170 R) It includes CO2 sensor, an automatic auto-zero programmable function to ensure accurate calibrated measurements. HEPA filtration of the gas supply inlets minimizes contamination risk. O2 control system (in 0.1-19%)  is also included. So, it is for cell incubation in all type of cell culture  specifically designed for  neuronal differentiation from iPS cells under low O2  regulation. 1
  Fluorescence Cell Counter (Luna FL,L20001) It is an image-based automated cell counter specifically designed to count primary cells such as iPS cell, neutrophil, and stem cells. Using the most advanced fluorescent optics and image analysis software, the Luna-FL™ accurately measures cell viability and cell number for fluorescently stained or trypan stained cells. 1
  Inverted Fluorescence Microscope
Nikon TE2000 with a high resolution cool CCD camera (DXM-1200C) and control software (ACT-1 and NIS-Elements D)
High-end inverted bright field and fluorescent microscope with motorized shutter, a high resolution cooled colour CCD camera, PC unit with controlling software. Observation, image capturing and image data analysis of bright field and fluorescence samples for culture cells. 1
  Single cuvette based transfection system (Nucleofector™ 2b Device, Lonza) It allows highly efficient transfection of hard-to-transfect cell lines and primary cells with different substrates such as  DNA vectors, siRNA and antisense oligonucleotides  in low-throughput format. 1
Hybrid Refrigerated Centrifuge (CAX-571, TOMY) High-performance refrigerated centrifuge that combines functions of high speed micro centrifuge and versatile low speed centrifuge into one compact model, meets a wide range of applications in one unit from micro tube rotors to multi-place swing rotors. 1

SPF Animal Facility

EquipmentModel IdentificationShort Description of CapabilitiesQuantity
 Rodent behaviour system
1. Open field test (Lime light 2.7, Actimetrics Inc)
2. Forced swimming test (Macro Act 1.06, Neuroscience Inc)
3. Light-Dark transition test (MED-PC IV, Med Association Inc)
4. Prepulse inhibition test system (SR-LAB, San Diego Instruments)
5. Activity sensor system (DAS system Mu 
Rodent behaviour system can be used for several behaviour study in rodent models. Anxiety-like behaviour (Open field test, Light transition test), Depressive behaviour (Force swimming test), Circadian rhythm, Sleep disorder (activity sensor), Schizophrenia-like behaviour and Learning and memory (Object placement test)1
 1. Stereotaxic Instrument (SRS-6 HT, Narishige Inc)
2. Micro drill
Stereotaxic surgery system can be used for microinjections into the target site of the brain or other tissue to deliver drugs, viral vectors or cell transplant. 1

TG Fish Facility

EquipmentModel IdentificationShort Description of CapabilitiesQuantity
 Zebrafish behaviour system (MDE GmbH, Analysis software Komi 2.0 written by Pictron Kft)This  system can be used for several behaviour study in Zebrafish. In particular, locomotors activity, place preference, Anxiety-like behavior, Social interactive behavior Learning and memory1
 zebrafish microinjection system (FemtoJet express (Eppendorf)A microinjection system is to inject small volume of solution. Microinjection is performed under a microscope with the aid of a micromanipulator which allows for small movement under high magnification. We can deliver substances such as vector, dye, protein and DNA/RNA into cells, eggs and tissues with this system.
It is useful to create transgenic animals.
1

Collaboration

Meiji University, Japan (Faculty of Agriculture)
The Jikei University School of Medicine, Japan
Toyama university, Japan (Faculty of Science)
Nara Medical University  Japan
Central Institute for Experimental Animal, Japan
University of Malaya (Faculty of Medicine)
Sun Yat-Sen University, China
Chinese Hong Kong University, Hong Kong

Industrial Partner

Neuroscience, Inc.
Clea Japan, Inc.
Central Institute for Experimental Animal, Japan
Cancer Research Malaysia

Charges / Booking

Contact Person

Tomoko Saga
tomoko.soga@monash.edu
+60355146233

Shogo Moriya
shogo.moriya@monash.edu
+60355146164

Location of Equipment

iPS cell facility  3-3-34 in BRIMS
SPF animal facility 3-8-7 - 3-8-12 in BRIMS
Fish facility 3-8-05 - 06 in BRIMS