PhD studentship (Dr. Kumaran Narayanan) (2)
Project Title: E. coli vector for gene delivery into mammalian cells.
Description of the project:
E. coli is the standard tool in molecular biology for manipulating, maintaining and propagating DNA, including its use as the host for human genomic libraries. This raises the exciting possibility of combining the great utility of E. coli as a cloning vehicle with its ability to deliver DNA, resulting in a potentially extremely powerful vector system for gene expression in mammalian cells. We have adapted a nonpathogenic E. coli strain to successfully deliver plasmids and artificial chromosomes of up to 200 kb into mammalian cells (Narayanan et al., 2013; Chen et al., 2014; Narayanan and Warburton, 2003). This PhD project will advance the development of this vector to optimize it for gene delivery into various mammalian cell types.
Chen, Q., Lee, C.W., Sim, E.U, and Narayanan, K. (2014) Induction of protein expression within E. coli bactofection vector for entry into mammalian cells. Human Gene Therapy Methods 25: 40-7.
Narayanan, K., Sim, E.U, Lee, C.W., Radu, A. (2013). E. coli bactofection using Lipofectamine. Analytical Biochemistry 439:142-4.
Narayanan, K., and Warburton, P.E. (2003). DNA modification and functional delivery into human cells using Escherichia coli DH10B. Nucleic Acids Res. 31, e51.
The candidate should hold a B.Sc (Hons) degree and MUST be a Malaysian citizen in order to be eligible for stipend support. Strong preference will be given to applicants with proven expertise in molecular biology/genetics who are highly motivated and proven to work independently with minimal supervision.
To apply, please email your CV and 2 letters of references (at least one must be from an immediate supervisor) explicitly addressing the criteria above. Only qualified candidates will be contacted to schedule for an interview.Email:firstname.lastname@example.org
For PhD requirements, please refer to this link: Doctor of Philosophy (PhD)
For English requirements, please refer to this link: Doctor of Philosophy (PhD)