Zooplankton Distribution in Face of Global Warming: Meta-Manscriptome Approach
Plankton are organisms that passively drift in water currents. However, each plankton species has its own biogeographic distribution. How do different populations and species of plankton achieve their biogeographic distributions within the open ocean, where water currents disperse and mix different populations and species? To answer this question, we are currently performing meta-transcriptomic analysis of zooplankton community samples. Using this method, we can determine nucleotide sequences of high-resolution (population level) marker regions without amplification bias from zooplankton community samples. In the face of global warming, it is also important that we are able to assess the spatial distribution of zooplankton species and populations in a limited time and with limited resources. We have collected zooplankton samples from several locations in the tropical to subtropical North Pacific. PolyA+ RNA was purified from total RNA, and cDNA libraries were constructed from the purified products. Illumina sequencing libraries were further constructed from the cDNA. Those libraries were sequenced by MiSeq (Illumina) at the Sequencing Core Facility of the Biodiversity Research Centre, Academia Sinica.
Dr. Ryuji Machida
- Assistant Research Fellow, Biodiversity Research Center, Academia Sinica, Taiwan
- Faculty Member, Taiwan International Graduate Program in Biodiversity (BIODIV)