Tan Hock Siew, Patrick

Lecturer
School of Science

tan.hocksiew@monash.edu
+603 5514 6116
Room 4-8-17
http://orcid.org/0000-0003-0794-6782

Dr Patrick Tan obtained his Honours degree from University Sains Malaysia in 2008 and went on to complete his Masters in Biotechnology in 2011. A year later, he secured a research fellowship to complete his doctoral work at the University of Wuerzburg in Germany. Dr Tan was trained in the field of microbial genetics and biotechnology. His doctoral studies focused on understanding the role of small RNAs (sRNAs) in Helicobacter pylori, a human pathogen that causes gastric ulcer.

His goal is to contribute to betterment of our society through life science research and education. Besides work, Dr Tan enjoys promoting STEM (science, technology, engineering, and mathematics) education to children and the public. He is also an avid photographer and a chess fan.

Qualifications

  • Doctor of Philosophy, Julius Maximilian University of Wuerzburg, Germany, 2017
  • Master in Biotechnology, Universiti Sains Malaysia, 2011
  • Bachelor of Applied Science (Hons) Biotechnology, Universiti Sains Malaysia, 2008

Expertise

RNA biology of bacteria, Microbial genetics, and Biotechnology

Research

My first research interest is to study the RNA biology of pathogens.  During infection, pathogens often encounter various environmental stresses which require rapid response. RNA-based regulation (riboregulation) is often used by bacteria because it is faster compared to protein-based regulation. The importance of riboregulation in pathogens has gained much attention in recent years. It is now accepted that extensive post-transcriptional control of gene expression in bacteria regulates many cellular processes including virulence and adaptation. I am interested in using global RNA biology approaches to characterise transcriptome profiles and uncover RNA-based control mechanisms of clinically relevant pathogens.

My second interest is to develop riboregulators as alternative targets for antimicrobials via RNA-based Synthetic Biology approaches. The prevalence of multidrug-resistant pathogens calls for the development of new targeted antimicrobials. Recent developments in the field of RNA biology have positioned RNAs as potential antimicrobial agents. Why RNA? These molecules are well-characterized, modular, and consume less cellular resources compared to proteins, making RNA an attractive substrate for bioengineering.

We are currently working on elucidating the antimicrobial response of Shigella spp. in vitro and in vivo using a combination of next generation sequencing (NGS), genetic engineering and biochemical approaches. For the latest updates on this project, please visit our research page at https://www.researchgate.net/lab/Patrick-HS-Tan-Lab-Hock-Siew-Tan.

For interest in potential collaborations and research projects (undergraduate and postgraduate), please contact me at the email address above.

Teaching

BIO1022 (Life on Earth)

BTH2732 (Recombinant DNA Technology)

Scholarly Journals

  • A small RNA decreases the sensitivity of Shigella sonnei to Norfloxacin. Gan IN, Tan HS, BMC Res Notes. 2019 12(1):97.
  • Identification of the RNA pyrophosphohydrolase RppH of Helicobacter pylori and global analysis of its RNA targets. Bischler T, Hsieh PK, Resch M, Liu Q, Tan HS, Foley PL, Hartleib A, Sharma CM, Belasco JG. J Biol Chem. 2017 292(5):1934-1950.
  • Differential RNA-seq (dRNA-seq) for annotation of transcriptional start sites and small RNAs in Helicobacter pylori. Bischler T, Tan HS, Nieselt K, Sharma CM. Methods. 2015 86:89-101.
  • Draft genome sequence of the rubber tree Hevea brasiliensis. Rahman AY, Usharraj AO, Misra BB, Thottathil GP, Jayasekaran K, Feng Y, Hou S, Ong SY, Ng FL, Lee LS, Tan HS, Sakaff MK, Teh BS, Khoo BF, Badai SS, Aziz NA, Yuryev A, Knudsen B, Dionne-Laporte A, Mchunu NP, Yu Q, Langston BJ, Freitas TA, Young AG, Chen R, Wang L, Najimudin N, Saito JA, Alam M. BMC Genomics. 2013 14:75
  • Tan, H.S., Lee, S.M., 2019, A Multi-omics Approach to Study the Evolution of Resistance during Low-Level Antibiotics Exposure in Shigella., Early Career Researchers Seed Fund Grant, Monash University Malaysia, RM20k
  • Tan, H.S., Ayub, Q, Rahman, S., Lee. S.M., 2019-2020, Functional Genomics Study on the Antimicrobial Response of Acinetobacter baumannii Strains Isolated From the Community and Hospital in Segamat, Global Asia in 21st century platform, Monash University Malaysia, RM20k
  • Tan, H.S., Ling, I., Dhanoa, A., 2019, Nucleic-acid-based strategy to treat antimicrobial resistant Enterobacteria, Tropical Medicine and Biology Multidisciplinary platform, Monash University Malaysia, RM10k
  • Tan, H.S., Ayub, Q., 2018, Identification of riboregulators in Shigella sonnei during antibiotic stress using Next-Generation Sequencing approach,Tropical Medicine & Biology Multidisciplinary platform, Monash University Malaysia, RM10k
  • Tan, H.S, Sharma, C., 2012-2015, PhD Research Fellowship, University of Wuerzburg, Germany.

PhD students (Present)

  1. Md Mainul Hasan Sarker (co-supervisor)
  2. Nazmul Hasan Muzahid (co-supervisor)
  3. Nooryasmin Anwar (co-supervisor)

Honours students (Present)

  1. Gan I-Ning
  2. Choo Earn
  3. Wong Bao Chi
  4. Ten Kah Ern

3rd year project students (Present)

  1. Looi Sue-Jen

3rd year project students (Completed)

  1. Gan I-Ning
  2. Choo Earn

Professional Association

German Society for Hygiene and Microbiology (Member, 2014 to present)

Malaysian Sociery fo Microbiology (Member, 2018 to present)

Academic/Corporate Community Engagements

International Presentations

  • 2016, Munich, Germany - Sensory and Regulatory RNAs in Prokaryotes.
  • 2014, Dresden, Germany - Fourth Joint Conference of the German Society for Hygiene and Microbiology & the Association for General and Applied Microbiology.
  • 2013, Wennigsen, Germany - Helicobacter Workshop.

Local and International Awards

2014

  • International Symposium Wuerzburg, University of Wuerzburg, Germany for the Best Poster Award for The role of an acid-responsive sRNA in Helicobacter pylori.

2008

  • Dean's list, School of Biological Sciences, Universiti Sains Malaysia

2007

  • Dean's list, School of Biological Sciences, Universiti Sains Malaysia