Yap Khai Khun, Michelle

Lecturer
School of Science

yap.michelle@monash.edu
+603 5514 5860
Room 4-8-39
https://orcid.org/0000-0002-9646-3944

Dr Michelle Yap earned her Bachelor of Science degree in 2006 followed by PhD in 2014 from University of Malaya focusing on the toxicology and pharmacology of bioactive proteins from medically important venomous species – an interest she still continue as an appointed lecturer within the School of Science. Here, she is also keen to investigate the proteomics profile of bioactive proteins and their immunogenicity, in addition to molecular modelling of proteins of interests. Her current research interest also focuses on molecular mechanisms of cytotoxicity of venom cytotoxin and the toxin-receptor interactions.

Qualifications

  • Doctor of Philosophy, University of Malaya, 2014
  • Bachelor of Science, University of Malaya, 2009

Expertise

  • Proteomics
  • Molecular modelling & protein-protein interactions
  • Toxicology & Pharmacology of bioactive proteins: Pharmacokinetics

Research Interest

  • Investigate the proteomics profile of bioactive proteins and their expression levels using Next Generation Sequencing (NGS), in addition to the phylogenetic analysis and molecular modelling of proteins of interests.
  • Molecular docking of toxin with receptors.

Teaching

  • BTH2752 (Cellular Metabolism)
  • BTH2741 (Biochemistry)
  • PHA3801 (Principles of Pharmacology)

Scholarly Journals

Tan CH, Tan KY, Yap MKK, Tan NH (2017). Venomics of Tropidolaemus wagleri, the sexually dimorphic temple pit viper: Unveiling a deeply conserved atypical toxin arsenal. Scientific Reports, 7:43237. doi:  10.1038/srep43237.

Fung SY, Tan CH, Yap MKK, Leong PK, Liew JL, Tan NH (2016). Unveiling the elusive and exotic: Venomics of the Malayan blue coral snake (Calliophis bivirgata flaviceps). Journal of Proteomics, 132:1-12.

Tan NH, Fung SY, Tan KY, Yap MKK, Ariaranee C, Tan CH (2015). Functional venomics of the Sri Lankan Russell’s viper (Daboia russelii) and its toxinological correlations. Journal of Proteomics, 128:403-423.

Yap MKK, Tan NH, Sim SM, Fung SY and Tan CH (2015). The effect of a polyvalent antivenom on the serum venom antigen levels of Naja sputatrix (Javan spitting cobra) venom in experimentally envenomed rabbits. Basic & Clinical Pharmacology & Toxicology, 117(4):274-279.

Yap MKK, Tan NH, Sim SM, Fung SY and Tan CH (2014). Pharmacokinetics of Naja sumatrana (Equatorial spitting cobra) venom and its major toxins in experimentally envenomed rabbits. PLOS NTD. DOI:10.1371/journal.pntd.0002890

Yap MKK, Fung SY, Tan KY and Tan NH (2014). Proteomic characterization of venom of the medically important Southeast Asian Naja sumatrana (Equatorial spitting cobra). Acta Tropica. 13: 15-25.

Yap MKK, Tan NH, Sim SM and Fung SY (2013). Toxicokinetics of Naja sputatrix (Javan spitting cobra) venom following intramuscular and intravenous administrations of the venom into rabbits. Toxicon. 68:18-23.

Yap MKK, Sim SM, Tan NH and Fung SY (2011). Pharmacokinetics of Naja sputatrix (spitting cobra) venom in rabbits. J Pharmacol Sci. 115 (Supplement 1): 183P-183P (ISI-cited Journal –Meeting abstract).

Yap MKK, Tan NH and Fung SY (2011). Biochemical and toxinological characterization of Naja sumatrana(Equatorial spitting cobra) venom. J Venom Anim Toxins incl Trop Dis. 17(4): 451-459.

Research Grants

Yap, M, 2018, Development of novel mammalian expression system for cobra cytotoxin, Faculty of Science Advancing Women Grant, RM10k

Yap, M, 2017, Purification, gene cloning and expression of cytotoxin in mammalian expression vectors., TMB Platform, RM5k

Yap, M, 2015, Comparative functional proteomics of the venoms of Wagler’s pit viper (Tropidolaemus wagleri), University of Malaya Research Grant (UMRG), RM43.5k

Yap, M, 2011, Pharmacokinetics of Naja sputatrix (Javan spitting cobra) and Naja sumatrana(Equatorial spitting cobra) venom and its proteomic studies, Postgraduate Research Grant (PPP), RM30k

Yap, M, 2010, Serum kinetics of Naja sputatrix Venom and Antivenoms, University of Malaya Research Grant, RM15k

PhD students (Present)

  1. Ang Boon Hong
  2. Chai Jun Ching
  3. Anshika Sharma

Honours students (Present)

  1. Ravini Srilal
  2. Nurhamimah Misuan

3rd Year Project students (Present)

  1. Yong Yanning
  2. Shusruto Rishik

3rd Year Project students (Completed)

  1. Liniaty
  2. Ewurama Dontoh

Professioal Association

Member of Professional Bodies

  • Malaysian Society of Biochemistry & Molecular Biology (Member, 4 years)
  • Malaysian Society on Toxinology, (Exco member, 3 years)

Academic/Corporate Community Engagements

Local Presentations

  • Shotgun analysis as rapid proteomics approach for identification of fermented soy (Glycine max) proteins. International Meeting & 42nd Annual Conference of the MSBMB in conjunction with the 3rd Joint International Symposium on Animal Cell Technology.
  • Drive safe, drive longer: Self-regulation is the key to road safety in older adults. International Forum of Quality and Safety in healthcare.
  • Biochemical and toxinological studies of Naja sumatrana (Equatorial spitting cobra) venom in 36th Annual Conference of Malaysian Society of Biochemistry and Molecular Biology, Malaysian Society of Biochemistry and Molecular Biology.
  • Serum kinetics of Naja sputatrix (spitting cobra) venom in rabbit and immunological responses in rabbit immunized with Naja sputatrix venom and venom components in 35th Annual Conference of Malaysian Society of Biochemistry and Molecular Biology, Malaysian Society of Biochemistry and Molecular Biology.
  • Isolation and purification of alpha-amylase inhibitor from Morinda citrifolia in 20th Intervarsity Biochemistry Seminar, Malaysian Society of Biochemistry and Molecular Biology.

International Presentations

  • Pharmacokinetics of Naja sputatrix (Javan spitting cobra) venom and the effect of a neuro polyvalent antivenom in 17th Congress of EU section of International Society of Toxinology, International Society of Toxinology.
  • Pharmacokinetics of Naja sputatrix (spitting cobra) venom in rabbits in The 11st Southeast Asian Western Pacific Regional Meeting of Pharmacologists, The Japanese Pharmacological Society.

Awards

2012

  • University of Malaya Fellowship Scheme
  • Best Poster Presentation, 35th Annual Conference of Malaysian Society of Biochemistry and Molecular Biology, Malaysian Society of Biochemistry and Molecular Biology

2011

  • 11st SEAWP-RFP Travel Fellowship Award for Young Scientist, Japanese Pharmacological Society

2008

  • Dean's list, Faculty of Science, University of Malaya