MS- A general LCMS scan. It will detect all compounds in your samples. Suitable for compound discovery. The downside is the m/z, and the matched compound name is not confirmed since one m/z can be shared with multiple compounds.
tMSMS (targeted MSMS)- For targeted compound confirmation based on the MS data, analyzed and mined via collision energy (CE) application on the parent compound ion. Collision energy fragmented parent ions into daughter/product ions. Common CE is 20V.
aMSMS (auto MSMS)- Compound conformation based on range of m/z (not on specific parent compound). The advantage is that it is cost-efficient as we only use one injection to get the daughter/product ions fragments. The downside is that it depends on the system's settings and the abundance of the parent ions. Low abundance ions might not be detected or lost.
