Library Prep & Sequencing

Library Preparation

DNA size selection

  • Using Covaris M220 Focused-Ultrasonicator™ (100 bp to 1,000 bp).
  • Using BluePippin with Pulsed-Field electrophoresis (100 bp to 50 kb).

DNA

  • Library preparation for Illumina, Oxford Nanopore Technologies and PacBio Sequel IIe  sequencing.
  • Targeted amplification for 16S ribosomal RNA (rRNA) (V3 & V4 regions or whole gene) metagenomics.
  • Targeted resequencing of genes, microsatellites and other variations.
  • Double digest Restriction Associated DNA (ddRAD) sequencing library preparation.
  • COI amplicon barcoding.

RNA

  • mRNA, poly-A enrichment.
  • Small RNA isolation.

Sample pre- & post- QC assessment

  • Bioanalyzer or Tapestation, RNA integrity (RIN).
  • Bioanalyzer or Tapestation, low concentration DNA/RNA length estimation.
  • Qubit, quantitative fluorescence of DNA/RNA.
  • Quantitative polymerase chain reaction (qPCR).

Sequencing Services

Our current in-house sequencing mainly utilizes the Illumina MiSeq platform. We also offer options for long-read sequencing using Oxford Nanopore Technologies and PacBio Sequel IIe high fidelity (HiFi) reads.

Below are the common sequencing application types we offer:

  1. Mitogenome (mitochondrial DNA) Sequencing.
  2. Whole Genome Sequencing (small genomes < 5 Mb).
  3. Amplicon Sequencing.
  4. Transcriptome Sequencing.
  5. Small RNA Sequencing.
  6. Metagenome Sequencing.
  7. ddRAD.
  8. Bisulfite Sequencing.